Contribution of Surface Salt Bridges to Protein Stability†,‡
نویسندگان
چکیده
منابع مشابه
Contribution of surface salt bridges to protein stability: guidelines for protein engineering.
The small globular protein, ubiquitin, contains a pair of oppositely charged residues, K11 and E34, that according to the three-dimensional structure are located on the surface of this protein with a spatial orientation characteristic of a salt bridge. We investigated the strength of this salt bridge and its contribution to the global stability of the ubiquitin molecule. Using the "double mutan...
متن کاملDefining the role of salt bridges in protein stability.
Although the energetic balance of forces stabilizing proteins has been established qualitatively over the last decades, quantification of the energetic contribution of particular interactions still poses serious problems. The reasons are the strong cooperativity and the interdependence ofnoncovalent interactions. Salt bridges are a typical example. One expects that ionizable side chains frequen...
متن کاملThe contribution of interchain salt bridges to triple-helical stability in collagen.
Studies on collagen and collagen-like peptides suggest that triple-helical stability can vary along the amino acid chain. In this regard, it has been shown that lysine residues in the Y position and acidic residues in the X' position of (GPO)(3)GXYGX'Y'(GPO)(3) peptides lead to triple-helical structures with melting temperatures similar to (GPO)(8) (where O is hydroxyproline), which is generall...
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We estimate the free energies of transfer of ionized amino acid side chains in water to both their ion-paired and neutral hydrogen-bonded states in low-dielectric media. The difference between the two free energies corresponds to the proton transfer free energy in a "salt bridge" formed between acidic and basic groups (i.e., lysine and glutamic acid residues). Our approach is to use gas phase p...
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absorbed in the atmosphere, the NIR water vapor bands. Thus, we see no way of defending Stephen's statement that conclusions based on broad-band measurements (1) are inconsistent with other existing data sets where discrepancies were found to occur between measurements in spectral bands and theory. Instead, the broad-band solar absorption findings presented in our report (1) are entirely consis...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2000
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi992257j